The Physcomitrella codon optimization tool for CDS sequences
physCO is a companion program of Top et al. (2021). The source code is available under AGPL v3.0 at https://doi.org/10.5281/zenodo.4905544.
The program uses codon information as described in Nakamura et al. (2000) Nucl. Acids Res. 28:292 and Hiss et al. (2017) Front. Plant Sci. 8:1842. If you publish a sequence optimized by physCO, please cite accordingly.
The program accepts CDS sequences in FASTA format, one at a time. It checks the input for valid alphabet, conclusive length and proper CDS codon structure and outputs a codon-optimized version along with some some basic log.
Try it: paste a single CDS sequence and hit "Optimize".